Journal: Cell Death & Disease
Article Title: Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signaling
doi: 10.1038/s41419-025-08248-x
Figure Lengend Snippet: A The protein mass spectrum of Wls were shown. B Fresh lung homogenates were sparked by anti-Flag or anti-Wls for Wls or SNX3 detection in co-IP analysis. C IHC staining analysis shown the Wls expression in BLM-induced mice (Scale bar: 200 μm; n = 8 mice). D The protein level and colocalization of Wls with SNX3 in Snx3-cTg mice were detected by IF staining analysis (Scale bar: 100 μm, n = 8 mice). E The protein level of Wls were detected by IHC staining analysis in Snx3-cTg mice (Scale bar: 200 μm; n = 8 mice). F The protein level and colocalization of Wls with SNX3 in Snx3-cKO mice were detected by IF staining analysis (Scale bar: 100 μm, n = 8 mice). G The protein level of Wls in Snx3-cKO mice were detected by western blotting analysis ( n = 8 mice). H , I IF staining analysis were performed to detected the protein level and colocalization of Wls with Rab 5a and LAMP1; Scale bar: 10 μm; n = 3 experiments. J The Wls intracellular recycling and degradation flow chart. Were created with BioGDP.com K H&E staining and PSR staining in lung tissue sections were shown. Scale bar: 200 μm, n = 8 mice. L Representative images of IHC staining analysis in Snx3-cKO mice were shown; Scale bar: 200 μm, n = 8 mice. M IF staining analysis of α-SMA were shown; Scale bar: 25 μm, n = 3 experiments. N IF staining analysis of β-catenin were shown; Scale bar: 25 μm, n = 3 experiments. The data were shown as means ± SEM. * P < 0.05 vs. CTL+ Saline group or sh-NC. # P < 0.05 vs CTL + BLM group or sh-NC + TGF-β1. & P < 0.05 vs Snx3-cKO + BLM group or sh-SNX3 + TGF-β1. ns, not significant.
Article Snippet: Then, cells were blocked with PLA blocking buffer for 1 h at 37 °C and incubated with rabbit anti-Wls (Proteintech, China, #23081-1-AP), anti-CK-1α (Abcam, USA, # EPR19824 ) and mouse anti-SNX3 (Santa Cruz, USA, diluted 1:200, #sc-376667) overnight at 4 °C.
Techniques: Co-Immunoprecipitation Assay, Immunohistochemistry, Expressing, Staining, Western Blot, Saline
Bioss is a verified supplier 
Bioss manufactures this product 
